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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$198.00

 

EC 3.4.21.4

SOURCE.  Rat Pancreas.

SPECIFICATIONS.  From autolyzed rat pancreas.  Chromatographically purified, lyophilized, salt-free.  E, 1%, 280 nm=14.50  MW= 25000

Store at 5°C.  Stable 9 to 12 months at 5°C.

ACTIVITY   15-20 units per mg of protein.  One unit will hydrolyze 1 µM of N-benzoyl-Phe-Val-Arg-pNA (EPC no. NB32) per minute at pH 8.3 and 25°C.  Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCl2 and 0.2 mM substrate. 

Use 1-5 µg enzyme in the assay.

Chymotrypsin; >0.1% of specific activity on the assay N-Suc-Al-Al-Pro-Phe-pNA (EPC No. SG554).  Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCl2 and 0.2 mM substrate.