Prepared from Elastin EPC No. E60 by proprietary modifications of the method of Mecham and Lange (5).
Generally, bovine neck ligament elastin is extracted two times for 60 minutes at 37º C with 1 M HaOH om 80% ethanol. The second extraction supernatant is neutralized with HC103, clarified by centrifugation, dialyzed and lophilized. ETNA-elastin is a salt-free, water soluble powder.