SOURCE. Leucocytes of Purulent Human Sputum.
Highly purified by ion exchange and gel diffusion chromatography.
Purity greater than 98% by PAGE and gel diffusion
Store at 5° C. Stable 1 year.
A lyophilized, salt-free, green soluble powder.
Absorbance ration 430nm/280 nm is 0.70 to 0.75 on 0.1 mg/ml in 0.05 M phosphate pH 7 buffer.
MW ca. 59,000 in SDS-PAGE indicating two protein subunits.
MW ca. 120,000 on S-200 chromatography.
150 -160 units per mg of protein.
1 unit will decompose 1 µmole of H2O2 per minute at pH 7.0 and
25°C using 4-amino-antipyrine as hydrogen donor. The assay procedure is given in Methods.