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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$675.00

SOURCE. From Human Lung.

 


DESCRIPTION.
Tryptase is a serine protease purified from human lung mast cells. The enzyme is prepared by ion-exchange and affinity chromatography. Purity is approximately 95% as determined by analytical gel diffusion on Superdex 200, native PAGE and reduced SDS-PAGE. The molecular weight is approximately comprising 4 subunits of approximately 3` to 37 Kd. Tryptase has recently been shown to be an active causative enzyme in asthma (1).

 

STABILITY. SolutionA; 1 mg per ml of tryptase in 0.05 M NaOAc pH5 + 1.0 M NaCl  + 0.01% NaN3.
Solution B; solution A diluted with 1 part glycerol.
At room temperature (21-23°C), solution A remained stable for 7 days. On day eight, 90% of initial specific activity remained. Solution A retained 95% of initial activity through 10 freeze thaw cycles (-20°C to room temperature) over a period of 15 days. Solution B retained 95% of initial activity through 12 temperature change cycles (-20°C to room temperature) over 25 days.

 


SPECIFICATIONS.
Supplied as 1 mg per ml in 0.05 M NaOAc pH 5 containing 1 M NaCl +0.01% NaN3.

Store at -20°C, stable 12 months.  Protein determined by method of Bradford using BSA as standard. (4)
55-70 units per mg of protein.
One unit will hydrolyze one µM of N-benzoyl-Phe-Val-Arg-pNA  (EPC No. NB32)per minute at pH 8.3 at 25°C.
 

 




References.

(1) Elrod, K., More, Wm., Am. H. Respir. Crit. Care Med. Bol 156 pp 375-381, 1997.

(2) Schwartz, L. Lewis, R., Austin, K., J. Biol. Chem., Vol. 256 pp. 11939-11943, 1981.

(3) Smith, T., Houglands, M., Johnson, D., J. Biol. Chem. Vol 259, pp 11046-11051, 1984.

(4)  Bradford, Marion, Analytical Biochemistry, 72,248-254 (1976). 

Note:

All human donor tissues are screened for the following:
Basic Serology Panel #1, RPR Syphilis Serology, ABO/Rh Typing HIV-1/HIV2 Antibody Screen, Hepatitis B Surface Antigen, Hepatitis B Core IgG/Igm Antibody, Hepatitis C Virus Antibody, HTLV-I Antibody. We accept only donors who are non reactive to the above screening.