SOURCE. From Human Lung.
Tryptase is a serine protease purified from human lung mast cells. The enzyme is prepared by ion-exchange and affinity chromatography. Purity is approximately 95% as determined by analytical gel diffusion on Superdex 200, native PAGE and reduced SDS-PAGE. The molecular weight is approximately comprising 4 subunits of approximately 3` to 37 Kd. Tryptase has recently been shown to be an active causative enzyme in asthma (1).
STABILITY. SolutionA; 1 mg per ml of tryptase in 0.05 M NaOAc pH5 + 1.0 M NaCl + 0.01% NaN3.
Solution B; solution A diluted with 1 part glycerol.
At room temperature (21-23°C), solution A remained stable for 7 days. On day eight, 90% of initial specific activity remained. Solution A retained 95% of initial activity through 10 freeze thaw cycles (-20°C to room temperature) over a period of 15 days. Solution B retained 95% of initial activity through 12 temperature change cycles (-20°C to room temperature) over 25 days.
Supplied as 1 mg per ml in 0.05 M NaOAc pH 5 containing 1 M NaCl +0.01% NaN3.
Store at -20°C, stable 12 months. Protein determined by method of Bradford using BSA as standard. (4)
55-70 units per mg of protein.
One unit will hydrolyze one µM of N-benzoyl-Phe-Val-Arg-pNA (EPC No. NB32)per minute at pH 8.3 at 25°C.
(1) Elrod, K., More, Wm., Am. H. Respir. Crit. Care Med. Bol 156 pp 375-381, 1997.
(2) Schwartz, L. Lewis, R., Austin, K., J. Biol. Chem., Vol. 256 pp. 11939-11943, 1981.
(3) Smith, T., Houglands, M., Johnson, D., J. Biol. Chem. Vol 259, pp 11046-11051, 1984.
(4) Bradford, Marion, Analytical Biochemistry, 72,248-254 (1976).
All human donor tissues are screened for the following:
Basic Serology Panel #1, RPR Syphilis Serology, ABO/Rh Typing HIV-1/HIV2 Antibody Screen, Hepatitis B Surface Antigen, Hepatitis B Core IgG/Igm Antibody, Hepatitis C Virus Antibody, HTLV-I Antibody. We accept only donors who are non reactive to the above screening.