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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$343.00

EC 3.4.21.4

 

SOURCE. Human pancreas.

 

SPECIFICATIONS.
From autolyzed human pancreas. Chromatographically purified, lyophilized; may contain not more than 2% NaCl.
Store at -20° C. Stable 9 to 12 months.

 

ACTIVITY.
25-30 Units per mg of trypsin protein.
One unit will hydrolyze 1 µM of N-benzoyl-Phe-Val-Arg-pNA(EPC No. NB32) per minute at pH 8.3 and 25° C.
Assay buffer is 100 mM Tris pH 8.3 containing 100 mM CaCl2 and 0.2 mM substrate. Use 0.5-2 µg enzyme in the assay.
E280 = 9.5.
Chymotrypsin; <0.1% of specific activity on the substrate Suc-Al-Al-Pro-Phe-pNA.(EPC No. SG554) Assay buffer is 100 mM Tris pH 8.3 containing 10 mM CaCl2 and 0.2 mM substrate.