Antigen: Human leucocyte elastase.
Lyophilized. Store at 4°C until rehydrated. Stable for a minimum of 1 year at 4°C. No preservatives have been added. Additional biological protection may be provided with 0.01% thimerosal. Non-sterile.
Rehydration and Storage:
Dilute to the volume indicated on the label with deionized water. Dilution with 50% glycerol, however, is preferred. This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to influence most assays.
Note: Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.
Tested by immunohistology as well as ELISA and Western blot techniques (5, 57). This product reacts most strongly with human neutrophil elastase. Cross-reactivity with elastase from non-human species has not been determined.
Suggested Working Dilutions:
Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations. Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Suggesting starting dilutions are as follows:
- Microwell Plate Assays 1:1000 - 1:2000
- Western blots 1:200 - 1:500
- Immunoprecipitation 1:100 - 1:200
- Histo/Cytochemistry 1:50 - 1:100