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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$164.00

EC 3.4.21.4

 

SOURCE. Rat pancreas.

 

SPECIFICATIONS.
From atuolyzed rat pancreas.
Chromatographically purified, lyophilized, salt-free.
E,1% 280= 14.50. MW-25,000

 

ACTIVITY.
15-20 units per mg of protein. One unit will hydrolyze 1 µmole of N-Benzoyl-Phe-Val-Arg-pNA (EPC No. NB32) per minute at pH 8.3 and 25°C. Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCI2 and 0.2 mM substrate. Use 1-5 µg enzyme in the assay.

 

Chymotrypsin;<.01% of specific activity on the substrate
Suc-Al-Al-Pro-Phe-pNA.(EPC No. FH237).
Assay buffer is 0.10 M Tris pH 8.3 containing 10 mM CaCl2and 0.2mM substrate.