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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$122.00

 

Elastin-rhodamine is prepared from Elastin (EPC No. E60) by labeling covalently with rhodamine-B-isothiocyanate. The particle size 200-400 mesh (75-37 micron).
Soluble peptides absorb maximally at 550 nm.
Fluorescence should be determined using excitation of 550 nm and emission of 570 nm.

 

The 200-400 mesh size is a sensitive substrate for the quantitative analysis of elastolytic activity. Elastolysis yields red-orange, highly fluorescent peptides that may be quantitated in a spectrophotometer or fluorometer. As little as 0.4 µgram of crystalline elastase per ml of incubation volume can be detected using the assay procedure described in Methods.

 

This elastin is insoluble in biological buffers.

 

  • Elastin Rhodamine, No. R144,Particle size: 200 - 400 Mesh (75 - 37 Micron)