Antigen: Human leucocyte Proteinase 3.
Whole serum obtained from rabbits immunized with purified human proteinase 3 (EPC no. ML734).
Tested by Western blot and immunoprecipitation.
Cross reactivity with proteinase 3 from non-human species has not been determined.
Lyophilized. Store at 4°C until rehydrated. Stable for a minimum of 1 year at 4°C. No preservatices have been added. Additional biological protection may be provided with 0.01% thimerosal. Non-sterile.
Rehydration and Storage:
Dilute to the volume indicated on the label with dionized water. Dilution with 50% glycerol, however, is preferred. This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to influence most assays. Note: Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.
Suggested working dilutions:
Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations. Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Suggesting starting dilutions are as follows:
- Microwell Plate Assays: 1:1000 - 1:2000
- Western Blot 1:200 - 1:500
- Immunoprecipitation 1:100 - 1:200
- Histo/Cytochemistry 1:50 - 1:100