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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$480.00

 Polylconal Antibody to Proteinase 3-IgG purified.

Antigen:  Human leucocyte Proteinase 3.

Host:  Rabbit

Form:  Protein A IgG purified and lyophilized.  0.01% NaN3 as a preservative.  Additional biological protection may be proviede with 0.01% thimerosal.  Non-Sterile.  Store at 4°C until rehydrated.  Stable for a minimum of 1 year at 4°C.

Rehydration and Storage:    Dilute to the volume indicated on the label with deionized water.  Dilution with 50% glycerol, however, is preferred.  This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C.  At most working dilutions, the level of glycerol is too small to influence most assays.

Note:  Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.

 

Suggested working dilutions:

Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations.  Prepare working dilution immediately before use.  Storage at a working dilution may result in performance loss.  Suggesting starting dilutions are as follows:

  • Microwell Plate Assays:          1:1000 - 1:2000
  •  Western Blot                              1:200 - 1:500
  •  Immunoprecipitation                1:100 - 1:200
  •  Histo/Cytochemistry                  1:50 - 1:100