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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$166.00

 

Antigen:  Porcine Pancreas Elastase

Host:  Rabbit

Whole serum obtained from rabbits immunized with purified porcine pancreatic elastase (EPC Product no. EC134).  Tested by ELISA.  Cross-reactivity with elastase from non-human species has not been determined.

Form:

Lyophilized.  Store at 4°C until rehydrated.  Stable for  a minimum of 1 year at 4°C.  No preservatives have been added.  Additional biological protection bay be provided with 0.01% thimerosal.  Non-sterile.

Rehydration and Storage:

Dilute to the volume indicated on the label with deionized water.  Dilution with 50% glycerol, however is preferred.  This procedure eliminates the need for freezing since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C.  At most working dilutions, the level of glycerol is too small to influence most assays.  Note:  Variations in termperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.

Suggested working dilutions:

Different assay conditions require that serial diluton of all reagents be performed to determine optimal working concentrations.  Prepare working dilution immediately before use.  Storage at a working dilution may result in performance loss.  Suggested starting dilutions are as follows:

  • Microwell Plate Assays                       1:100 - 1:200
  • Western blots                                        1:200 - 1:500
  • Immunoprecipitation                            1:100 - 1:200
  • HIsto/Cytochemistry                              1:50 - 1:100