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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$74.00

SOURCE. Porcine Pancreas. 
Prepared by chromatography, lyophilized. 
Contains predominately trypsin and chymotrypsin. 
Contains ca 0.2% elastase. 
White powder, highly soluble in 0.01 M HCl, >75 mg/ml.

 

 

Trypsin activity 17.0 to 20.0 units per mg of solid.

 

Chymotrypsin activity 16 to 19 units per mg of solid.

 

Assays done in 0.1 M Tris pH 8.3 containing 0.15 mM N-benzoyl-Phe-Val-Arg-pNA (Trypsin)(EPC No. NB32) or 0.15 mM N-succinyl-Ala-Ala-Pro-Phe-pNA (chymotrypsin)(EPC No. SG554) and 2 to 5 mg protease.

 

One unit will hydrolyze 1 µM substrate per minute at 25°C.

 

Protease assay on casein. 
Substrate-buffer is 0.1 M Tris pH 7.5 containing 12.5 mg per ml of solubilized casein. One unit will hydrolyze 1 mg of casein per minute at 37°C. Protease activity >130 units per mg of solid.

 

Store 5°C. Stable 1-2 years.