• Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632

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Antigen: Mouse pancreatic elastase
Host: Rabbit
Lyophilized. Store at 4°C until rehydrated. Stable for a minimum of 1 year at 4°C. No preservatives have been added. Additional biological protection may be provided with 0.01% thimerosal. Non-sterile.
Rehydration and Storage:
Dilute to the volume indicated on the label with deionized water. Dilution with 50% glycerol, however, is preferred. This procedure eliminates the need for freezing, since 50% glycerol is an effective biological inhibitor when the product is stored at 4°C. At most working dilutions, the level of glycerol is too small to influence most assays.
Note: Variations in temperature due to freeze-thaw cycles may cause loss of activity when rehydrated product is stored in aliquots less than 50µl.
Suitable for Western blotting, ELISA, and Immunocytochemistry techniques.
Suggested Working Dilutions:
Different assay conditions require that serial dilution of all reagents be performed to determine optimal working concentrations. Prepare working dilution immediately before use. Storage at a working dilution may result in performance loss. Suggesting starting dilutions are as follows:

  • Microwell Plate Assays 1:1000 - 1:2000
  • Western blots 1:200 - 1:500
  • Immunoprecipitation 1:100 - 1:200
  • Histo/Cytochemistry 1:50 - 1:100