SOURCE. Porcine kidney.
Chromatographically purified, lyophilized, water soluble powder.
MW approximately 95,000
Store at -20°C. Will remain stable for 6 to 8 months at -20°C.
850-900 units per mg of protein.
One unit will liberate 1 µmole of pNA/min/mg protein from Glutaryl-(Ala)2-Phe-pNA (EPC No. GR511), at pH 7.4 and
Prepared by the modifications of the method of Gafford, J. (58).
Neutral endopeptidase (NEP; also known as kidney microvillus proteinase, neprilysin, enkephalinase, CD10 and CALLA [common acute lymphocytic leukemia antigen]) is a cell-surface, zinc-containing metallopeptidase that is present in many mammalian tissues including brain, liver, lung and endothelial cells. It is particularly abundant on the brush-border membranes of the kidney proximal tubule. NEP is a peptidase which has a specificity directed toward cleavage on the amino side of hydrophobic residues. In addition, an active site arginine on the enzyme can interact with the C-terminal carboxylate of a substrate.
Greater than 98% purity by HPLC (reverse phase).