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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$221.00

EC 3.4.24.11

 

SOURCE. Porcine kidney.

 

SPECIFICATIONS.
Chromatographically purified, lyophilized, water soluble powder.

 

MW approximately 95,000

 

Store at -20°C. Will remain stable for 6 to 8 months at -20°C.

 

850-900 units per mg of protein.
One unit will liberate 1 µmole of pNA/min/mg protein from Glutaryl-(Ala)2-Phe-pNA (EPC No. GR511), at pH 7.4 and
25°C.

 

PREPARATION.
Prepared by the modifications of the method of Gafford, J. (58).
Neutral endopeptidase (NEP; also known as kidney microvillus proteinase, neprilysin, enkephalinase, CD10 and CALLA [common acute lymphocytic leukemia antigen]) is a cell-surface, zinc-containing metallopeptidase that is present in many mammalian tissues including brain, liver, lung and endothelial cells. It is particularly abundant on the brush-border membranes of the kidney proximal tubule. NEP is a peptidase which has a specificity directed toward cleavage on the amino side of hydrophobic residues. In addition, an active site arginine on the enzyme can interact with the C-terminal carboxylate of a substrate.

 

Greater than 98% purity by HPLC (reverse phase).