SOURCE. Leucocytes of Purulent Human sputum.
SPECIFICATIONS.A lyophilized, salt-free, green soluble powder. Highly purified by ion exchange and gel diffusion chromatography. Purity 95% by PAGE and gel diffusion.
Store at 5°C. Stable 1 year.
Absorbance ration 430 nm/280 nm is 0.50 to 0.65 on 0.1 mg/ml in 0.05 M phosphate pH 7 buffer.
MW = 59000 in SDS-PAGE indicating two protein subunits.
MW = 12000 on S-200 chromatography.
75 - 90 units per mg of protein
1 unit will decompose 1 µM of H2O2 per minute at pH 7.0 and 25°C using 4-amino-antipyrine as hydrogen donor. Assay procedure is described in Methods.