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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$11,662.00

SOURCE. Human skin.

 

95% pure by PAGE.

 

DESCRIPTION.

Prepared by modification of Schwartz (83).

Chymase is a serine protease purified from human skin mast
cells. Purity is approximately 95% by analytical gel diffusion on Superdex 200
and SDS-PAGE.

 

MW - 30000

 

SPECIFICATIONS.

In 0.05 M NaOAc pH 5 + 1 M NaCl.

 

Store frozen at -20°C.

Stable 12 months at -20°C.

95 - 125 units per mg of protein.

Protein determined by coomasie blue assay (Bradford).

 

One unit will hydrolyze 1.0 mM of N-Benzoyl-L-Tryosine-Ethyl-Ester
(BTEE) per minute at pH 8.0 and 25°C.
Assay buffer is 0.30 M Tris pH 7.8 containing 1.5 M NaCl and 15% ethanol.
Use 1 to 3mg of enzyme.

 

Assay procedure described in Methods.