SOURCE. Human skin.
95% pure by PAGE.
Prepared by modification of Schwartz (83).
Chymase is a serine protease purified from human skin mast
cells. Purity is approximately 95% by analytical gel diffusion on Superdex 200
MW - 30000
In 0.05 M NaOAc pH 5 + 1 M NaCl.
Store frozen at -20°C.
Stable 12 months at -20°C.
95 - 125 units per mg of protein.
Protein determined by coomasie blue assay (Bradford).
One unit will hydrolyze 1.0 mM of N-Benzoyl-L-Tryosine-Ethyl-Ester
(BTEE) per minute at pH 8.0 and 25°C.
Assay buffer is 0.30 M Tris pH 7.8 containing 1.5 M NaCl and 15% ethanol.
Use 1 to 3mg of enzyme.
Assay procedure described in Methods.