SOURCE. Mouse Pancreas
Prepared by chromatography and 2x crystallized.
Lyophilized in a solution of 0.05 M Tris 7.5 containing 5 mM CaCl2 and 5 mM NaCl.
Each 0.1 mg contains about 0.35 mg salts. Re-dissolve with H2O.
Protein determined by Bradford.
Store - 20°C. Stable 12 months.
950 - 1000 units per mg of protein.
One unit ill liberate 1 µM of reducing groups equivalent to maltose per minute at pH 7.5 and 25° C. Substrate is soluble starch. Assay details, refer to Methods in the EPC Biochemical Catalogue.