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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$94.00

EC 3.4.21.36

 

SOURCE> Porcine pancreas.

 

LOW ENDOTOXIN; < 0.78 EU/mg.
A further purification of EPC No. EC134 to remove endotoxin by chromatography on imobilized polymyxin B and then filtered on 0.2 µ filter.

 

SPECIFICATIONS.
Chromatographically purified, salt-free, lyophilized water soluble powder. Free of trypsin, chymotrypsin, lipase, alpha amylase, and the carboxypeptidases.
MW = 25,500
Store 5°C. Stable 9-12 months with no decrease in activity.

 

ASSAYS.
115-125 units per mg of protein on the substrate elalstin orcein (EPC No. E154).
One unit will solubilize 1 mg of Elastin orcein in 20 minutes at pH 8.8 and 37°C.
Greater than 12 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA (EPC No. NS945).
One unit will hydrolyze 1µM per minute at pH 8.3 and 25°C. Assay procedures are described in Methods.

 

 

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