SOURCE> Porcine pancreas.
LOW ENDOTOXIN; < 0.78 EU/mg.
A further purification of EPC No. EC134 to remove endotoxin by chromatography on imobilized polymyxin B and then filtered on 0.2 µ filter.
Chromatographically purified, salt-free, lyophilized water soluble powder. Free of trypsin, chymotrypsin, lipase, alpha amylase, and the carboxypeptidases.
MW = 25,500
Store 5°C. Stable 9-12 months with no decrease in activity.
115-125 units per mg of protein on the substrate elalstin orcein (EPC No. E154).
One unit will solubilize 1 mg of Elastin orcein in 20 minutes at pH 8.8 and 37°C.
Greater than 12 units per mg of protein on the substrate Suc-Ala-Ala-Ala-pNA (EPC No. NS945).
One unit will hydrolyze 1µM per minute at pH 8.3 and 25°C. Assay procedures are described in Methods.