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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$71.00

 

Prepared from calf skin collagen (EPC Product No. C857) by labeling wth fluorescein-isothicyanate (isomer 1).

 

Excess FITC is removed by gel filtration and sequential precipitations with NaCl and dialysis (43).

 

3-4 mg collagen fluorescein per ml.

 

The collagen fluorescein is dissolved 0.1 to 4 mg/ml in 0.02 M aceitic acid at 5°C for 18 � 24 hours. Then collagen fluorescein is dialyzed at 5°C vs. 0.1 M Tris pH 7.5 containing 0.2 M NaCl. We follow the procedure of Baici et al., Anal. Biochem., Vol 108, page 230 (1980).

 

Store at 5°C. DO NOT FREEZE