SOURCE. Porcine pancreas.
2X crystallized, lyophilized powder containing approximately 65% protein and 35% sodium carbonate. MW = 25,500.
Free of trypsin, chymotrypsin, other pancreatic enzymes and euglobins.
Store at 5°C. Stable 2 years.
70-80 units per mg of powder on the substrate elastin-orcein.(EPC No. E154). The assay procedure is described in Methods.
One unit will solubilize 1 mg of elastin in 20 minutes at pH 8.8 and 37°C (11).
Greater than 4 units per mg of protein on the
substrate Suc-(Al)3-pNA. One unit will hydrolyze 1 µmole per minute at pH 8.3 and 25°C. Assay procedure described in methods.
Prepared from Elastase No. ES438.