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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$147.00

 

Elastin, No. E70 and ES70 are prepared by the method of Starcher et al. (3) wherein the bovine neck ligament is extracted with non-degradative reagents chosen specifically to minimize peptide bond cleavage.

 

In the method of preparation, finely divided ligament is extrated with saline-EDTA at pH 7.6, autoclaved, incubated with trypsin at pH 8.2, extracted with CNBr in formic acid and extracted with 6 M urea containing 2-Mercaptoethanol. (Washing with distilled water is required between each step. The final product is de-fatted and milled. This elastin is insoluble in biological buffers.

 

Elastin purified by this method was reported to be free of N-terminal amino acids. Our assay using a dansyl chloride method (4) indicates that the elastin is free of peptide bond cleavage. Additionally, only 0.005% insoluble residue remains after elastolysis.

 

    Elastin EPC No. E70,
  • Particle Size: 100-400 Mesh (149-37 Micron)

     

      Elastin EPC No. ES70,
    • Particle Size: Pass 400 Mesh (Smaller than 37 Micron)