SOURCE. Porcine pancreas.
2X crystallized, lyophilized powder containing approximately 90% protein and 10% sodium carbonate, MW = 25,500. Essentially free of protease. Contains euglobin proteins.
Store at 5°C. Stable 2 years.
65-85 units per mg of powder.
70-95 units per mg of protein.
One unit will solubolized 1 mg of elastin in 20 minutes at pH 8.8 and 37°C (11) using elastin-orcein as the substrate.
Greater than 4 units per mg of protein on the substrate Suc(Ala)3-pNA.
One unit will hydrolyze 1 µmole per minute at pH 8.3 and 25°C. Assay procedure described in Methods.
Prepared from Elastase, EPC No. E134. Has been used for isolation of type II lung cells.