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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$643.00

SOURCE. Human pancreas.

 


SPECIFICATIONS.

Chromatographically purified.

Lyophilized powder contains ca. 50% solids, comprising pH 6 buffer salts and sodium cholate. MW = 310000.

55-65 units per mg of protein.

One unit will hydrolyze 1 µmole of cholesterol acetate per minute at pH 7 at 37°C.

 


ASSAY BUFFER.

3ml KPO4 pH 7.0 containing 0.11 M NaCholate, 0.70 mM cholesterol acetate, 0.75 mM 4-aminoantipyrine, 4.8 mM Phenol, 15 units horseradish peroxidase and 0.30 units of cholesterol oxidase.

 


PROCEDURE.

Equilibrate 3.0 ml of assay buffer to 37°C in a 10 mm cuvette. Add .01 - .03 units CE. Read increase in A505 starting 2 minutes after the addition of CE.
E for 1 mM hydrolyzed cholesterol acetate = 6.8

 

Available quantities.

 

  • 0.1mg $400.00