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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$76.00

 

SOURCE. CI Histolyticum.

 

SPECIFICATIONS.
Chromatographically purified, salt-free, lyophilized water soluble powder.

 

Store at 5° C. Will remain stable 9 to 12 months at 5° C.

 

1000-2000 units per mg of protein. One unit will liberate a ninhydrin color equivalent to 1 µM of L-leucine at pH 7.5 in 5 hours at 37°C. Bovine achilles tendon collagen (EPC No. C204) is the substrate.

 

Also, 8-12 FALGPA units per mg of protein where 1 unit will hydrolyze 1 µmole of furanacryloyl-Leu-Gly-Pro-Ala at pH 7.5 per minute. (53).

 

Clostripain; <0.1 units per mg of protein

 

Tryptic Activity: <0.3 units per mg of protein.

 

Protease Activity: <1 units per mg of Protein.

 

PREPARATION.
Prepared by our modifications of the Keller and Mandl (20). In the method, crude collagenase is chromatographed sequentially on SP-Sephadex* at pH 6.0 and on DEAE-Cellulose at pH 8.0 These ion exchange steps remove most of the non-specific protein. The high collagenolytic fraction (low protease activity) is further purified by gel diffusion on Sephadex-G200. Non-specific protease is removed by affinity chromatography. Salt-free, lyophilized.