SPECIFICATIONS. Chromatographically purified from human pancreas.
Frozen solution in 0.05M NaOAc pH 5.0 + 1.0 M NaCl.
50 - 55 units per mg of protein.
Store frozen at approximately -20°C.
Concentration (A280 x 1.45)-(A260 x 0.74) = mg/ml.
One unit will hydrolyze 1 µmole of hippuryl-L-arginine per minute at pH 7.7 at 25°C.
The substrate buffer is 0.025 M Tris pH 7.7, 0.1M NaCl, 1 mM hippuryl-L-arginine.
E= 0.36 of 1 mM HLA.