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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$46.00

 

Soluble from calf skin, Type I, Lyophilized.

 

Prepared according to the method of Gallop and Seifter (21).

 

In the method, fresh calf skin is extracted with 0.5 M NaOAc to remove non-collagen proteins. The soluble collagen is extracted with 0.075 M sodium citrate pH 3.7 and precipitated as fibrils by dialysis against 0.02 M Na2HPO4.

 

Soluble in 0.01 M to 0.5 M acetic acid, maximum 10 mg/ml.

 

Soluble calf skin collagen is suitable for gel formation and assay of collagenase.

 

Collagen is soluble in 0.075 M sodium citrate pH 3.7 and in dilute acetic acid 0.01 M to 0.5 M.