SOURCE. Porcine pancreas.
SPECIFICATIONS. 3X crystallized. Soluble powder containing 15% sucrose as stabilizer. Also contains approximately 0.5% salts; Tris and CaCl.
Store at 5°C. Stable 9-12 months.
MW approximately 52,000
1500-1800 units per mg of protein.
1200-1450 units per mg of solid.
One unit will liberate 1 µM of reducing groups equivalent to maltose per minute at pH 6.9 and 25°C.
Substrate is soluble starch.
Contains less than 0.01% trypsin activity on the substrate BAPNA.
PREPARATION. Prepared by dialysis and lyophization of 3X crystallized alpha-amylase (EPC No. A663).