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  • Elastin Products Company, Inc.
    P.O. Box 568
    Owensville, Missouri, USA 65066

  • Phone: 573-437-2193
    Fax: 573-437-4632
    email: epc@fidnet.com

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$206.00

 

SOURCE. Porcine pancreas.

 

SPECIFICATIONS. 3X crystallized, suspension in 0.5 saturated NaCl pH 7.0 containing 3 mM CaCl2 and 0.01% NaN3 as preservative.

 

MW = 52,000

 

Store at 5°C. Stable 9-12 months at 5°C. DO NOT FREEZE.

 

1500-1800 units per mg of protein. One unit will liberate 1µM of reducing groups equivalent to maltose per minute at pH 6.9 and 25°C.
Substrate is soluble starch.

 

Contains less than 0.01% of trypsin activity on the substrate BAPNA.

 

PREPARATION. Alpha-amylase is three times crystallized by our modifications of the method of Levitzki et al. (29). In the method, the alpha-amylase of porcine pancreas is preferentially absorbed to glycogen and separated as a precipitate from the non-specific protein. After digestion of the precipitate, impurities are removed by chromatography and the alpha-amylase crystallized.
Trace proteases are removed by affinity chromatography.